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Image Search Results
Journal: Journal of cell science
Article Title: Loss of Ca 2+ entry via Orai-TRPC1 induces ER stress, initiating immune activation in macrophages.
doi: 10.1242/jcs.237610
Figure Lengend Snippet: Fig. 5. Tunicamycin, brefeldin A and SKF96365 treatment induce autophagy and apoptosis in Raw 264.7 macrophages. (A) Representative immunoblot images showing expression of markers for the induction of autophagy and apoptosis in Raw 264.7 macrophages treated with 10 µM Tuni, BFA or SKF for 12 h. (B) Quantification of densitometric values (mean±s.d.) from bands as shown in A. (C) Representative microscopy images of Raw 264.7 macrophages stained for LC3B (green), CHOP (red) and DAPI (blue) after cells were exposed to 10 µM Tuni, BFA or SKF for 12 h. **P<0.01; ***P<0.001 by one-way ANOVA test. The data shown are representative of three independent experiments. Scale bars: 20 µm.
Article Snippet: Thereafter, the slides were washed again, and coverslip mounted using
Techniques: Western Blot, Expressing, Microscopy, Staining